diff --git a/lib/internal/blob.js b/lib/internal/blob.js
index f16e520e197cdd..fc029674eb579f 100644
--- a/lib/internal/blob.js
+++ b/lib/internal/blob.js
@@ -530,6 +530,9 @@ function createBlobReaderStream(reader) {
         pending.reject(reason);
       }
       this.pendingPulls = [];
+      // Explicitly clear the reader wakeup callback to sever the GC reference
+      // between the active C++ handle and the pending JS stream.
+      reader.setWakeup(undefined);
     },
   // We set the highWaterMark to 0 because we do not want the stream to
   // start reading immediately on creation. We want it to wait until read