When using stage scan, the acquistion goes wrong in three places:
- In the first slice, piezo is defocused.
- Approximately in the middle of the stack, the stage seems to get shifted in the opposite direction of the scan.
- After the acquisition, when switching on live mode, the piezo seems to have returned to the origin position.
See this link for an example stack: https://cloud.mpi-cbg.de/index.php/s/TtcxVuyjBh7zqGs
When using stage scan, the acquistion goes wrong in three places:
See this link for an example stack: https://cloud.mpi-cbg.de/index.php/s/TtcxVuyjBh7zqGs