diff --git a/bindings/java/src/test/java/org/apache/opendal/test/behavior/BehaviorExtension.java b/bindings/java/src/test/java/org/apache/opendal/test/behavior/BehaviorExtension.java
index 3ad216a6acf9..18c4ba3ff26e 100644
--- a/bindings/java/src/test/java/org/apache/opendal/test/behavior/BehaviorExtension.java
+++ b/bindings/java/src/test/java/org/apache/opendal/test/behavior/BehaviorExtension.java
@@ -23,6 +23,7 @@
 import io.github.cdimascio.dotenv.DotenvEntry;
 import java.io.PrintWriter;
 import java.io.StringWriter;
+import java.nio.file.Paths;
 import java.util.HashMap;
 import java.util.Map;
 import java.util.UUID;
@@ -59,10 +60,18 @@ public void beforeAll(ExtensionContext context) {
                 }
             }
 
-            // Use random root unless OPENDAL_DISABLE_RANDOM_ROOT is set to true.
+            // Isolate each test class unless OPENDAL_DISABLE_RANDOM_ROOT is set to true.
             if (!Boolean.parseBoolean(dotenv.get("OPENDAL_DISABLE_RANDOM_ROOT"))) {
-                final String root = config.getOrDefault("root", "/") + UUID.randomUUID() + "/";
+                final String fixtureId = UUID.randomUUID().toString();
+                final String root = config.getOrDefault("root", "/") + fixtureId + "/";
                 config.put("root", root);
+                if ("sled".equalsIgnoreCase(scheme)) {
+                    final String datadir = config.get("datadir");
+                    if (datadir != null && !datadir.isEmpty()) {
+                        // Sled locks its physical database; logical roots do not isolate database handles.
+                        config.put("datadir", Paths.get(datadir, fixtureId).toString());
+                    }
+                }
             }
 
             @Cleanup
diff --git a/bindings/java/src/test/java/org/apache/opendal/test/behavior/SledFixtureIsolationTest.java b/bindings/java/src/test/java/org/apache/opendal/test/behavior/SledFixtureIsolationTest.java
new file mode 100644
index 000000000000..b582b21c617c
--- /dev/null
+++ b/bindings/java/src/test/java/org/apache/opendal/test/behavior/SledFixtureIsolationTest.java
@@ -0,0 +1,51 @@
+/*
+ * Licensed to the Apache Software Foundation (ASF) under one
+ * or more contributor license agreements.  See the NOTICE file
+ * distributed with this work for additional information
+ * regarding copyright ownership.  The ASF licenses this file
+ * to you under the Apache License, Version 2.0 (the
+ * "License"); you may not use this file except in compliance
+ * with the License.  You may obtain a copy of the License at
+ *
+ *   http://www.apache.org/licenses/LICENSE-2.0
+ *
+ * Unless required by applicable law or agreed to in writing,
+ * software distributed under the License is distributed on an
+ * "AS IS" BASIS, WITHOUT WARRANTIES OR CONDITIONS OF ANY
+ * KIND, either express or implied.  See the License for the
+ * specific language governing permissions and limitations
+ * under the License.
+ */
+
+package org.apache.opendal.test.behavior;
+
+import static org.assertj.core.api.Assertions.assertThat;
+import static org.junit.jupiter.api.Assumptions.assumeTrue;
+import org.apache.opendal.Capability;
+import org.junit.jupiter.api.Test;
+import org.junit.jupiter.api.condition.DisabledIfEnvironmentVariable;
+import org.junit.jupiter.api.condition.EnabledIfEnvironmentVariable;
+import org.junit.jupiter.api.extension.RegisterExtension;
+
+@EnabledIfEnvironmentVariable(named = "OPENDAL_TEST", matches = "(?i)sled")
+@DisabledIfEnvironmentVariable(named = "OPENDAL_DISABLE_RANDOM_ROOT", matches = "(?i)true")
+class SledFixtureIsolationTest extends BehaviorTestBase {
+    @RegisterExtension
+    static final BehaviorExtension anotherFixture = new BehaviorExtension();
+
+    @Test
+    void testIndependentFixtures() {
+        final Capability capability = op().info.capability;
+        assumeTrue(capability.read && capability.write);
+
+        final String path = "fixture-isolation";
+        final byte[] first = {1};
+        final byte[] second = {2};
+        op().write(path, first);
+        anotherFixture.operator.write(path, second);
+        assertThat(op().read(path)).isEqualTo(first);
+        assertThat(anotherFixture.operator.read(path)).isEqualTo(second);
+        op().delete(path);
+        anotherFixture.operator.delete(path);
+    }
+}