diff --git a/easystacks/software.eessi.io/2025.06/eessi-2025.06-eb-5.4.0-2025b.yml b/easystacks/software.eessi.io/2025.06/eessi-2025.06-eb-5.4.0-2025b.yml
index 57a4dcff35..086373102c 100644
--- a/easystacks/software.eessi.io/2025.06/eessi-2025.06-eb-5.4.0-2025b.yml
+++ b/easystacks/software.eessi.io/2025.06/eessi-2025.06-eb-5.4.0-2025b.yml
@@ -6,3 +6,4 @@ easyconfigs:
   - HMMER-3.4-gompi-2025b.eb
   - STAR-2.7.11b-GCC-14.3.0.eb
   - OpenMM-8.5.2-foss-2025b.eb
+  - Jellyfish-2.3.1-GCC-14.3.0.eb
\ No newline at end of file