diff --git a/easystacks/software.eessi.io/2025.06/eessi-2025.06-eb-5.4.0-2025b.yml b/easystacks/software.eessi.io/2025.06/eessi-2025.06-eb-5.4.0-2025b.yml
index 023af5374d..46bf44692c 100644
--- a/easystacks/software.eessi.io/2025.06/eessi-2025.06-eb-5.4.0-2025b.yml
+++ b/easystacks/software.eessi.io/2025.06/eessi-2025.06-eb-5.4.0-2025b.yml
@@ -2,3 +2,4 @@ easyconfigs:
   - IRkernel-1.3.2-gfbf-2025b-R-4.5.2.eb
   - buildenv-default-lfoss-2025b.eb
   - BEDTools-2.31.1-GCC-14.3.0.eb
+  - Bowtie2-2.5.4-GCC-14.3.0.eb